VE-Cadherin 蛋白, Mouse (HEK293, His)

VE-cadherin is a calcium-dependent glycoprotein critical for mediating cell adhesion and coordinating morphogenetic events during development. Lack of VE-cadherin in mice leads to uterine death due to increased endothelial cell apoptosis leading to vascular insufficiency. VE-Cadherin Protein, Mouse (HEK293, His) is the recombinant mouse-derived VE-Cadherin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of VE-Cadherin Protein, Mouse (HEK293, His) is 568 a.a., with molecular weight of approximately 85 kDa.

VE-钙粘蛋白是钙粘蛋白家族的成员，是一种钙依赖性糖蛋白，对于介导细胞粘附和协调发育中的各种形态发生事件至关重要。编码的前蛋白原经过加工产生成熟的功能蛋白。在小鼠中，VE-钙粘蛋白的缺失会导致子宫内死亡，这主要是由于内皮细胞凋亡加剧导致血管功能不全。值得注意的是，钙粘蛋白家族中的多个不同基因（包括该基因）位于 8 号染色体上。表达谱显示偏向某些组织，在肺和皮下脂肪垫等中观察到显着水平，强调了其在组织特异性中的重要性功能。

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 7.704 μg/mL, corresponding to a specific activity is 1.298×10² units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 7.704 μg/mL, corresponding to a specific activity is 1.298×10² units/mg.

Mouse

HEK293

C-10*His

NP_033998.2 (G25-A592)

12562  [NCBI]

GPNFPQIDTPNMLPAHHRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEFAGKIFGVDANTGNVLAYERLDREKVSEYFLTALIVDKNTNKNLEQPSSFTVKVHDINDNWPVFSHQVFNASVPEMSAIGTSVIRVTAVDADDPTVAGHATVLYQIVKGNEYFSIDNSGLIFTKIKNLDREKQAEYKIVVETQDALGLRGESGTATVMIRLEDINDNFPVFTQSTYTFSVPEDIRVGKPLGFLTVVDPDEPQNRMTKYSIMQGEYRDTFTIETDPKRNEGIIKPTKSLDYEVIQQYTFYIEATDPTIRYEYLSSTSGKNKAMVTINVLDVDEPPVFQRHFYHFKLPENQKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQFFRITKQGNIYNEKELDRETYAWYNLTVEANELDSRGNPVGKESIVQVYIEVLDENDNPPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNPKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHAKFHYLPVLISDNGVPSLTGTSTLTVGVCKCNEQGEFTFCEEMAA

approximately 85 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.