1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-C
  6. VEGF-CC 蛋白, Mouse/Rat (HEK293, His)

VEGF-CC 蛋白, Mouse/Rat (HEK293, His)

目录号: HY-P74474
COA 产品使用指南

VEGF-CC 蛋白刺激细胞增殖、迁移和血管通透性,这对于血管生成和内皮细胞动力学至关重要。它在胚胎发生过程中静脉和淋巴管系统的发育以及成人淋巴管内皮的维持中起着至关重要的作用。VEGF-CC 蛋白, Mouse/Rat (HEK293, His) 是重组的 VEGF-CC 蛋白,由 HEK293 表达,带有 C-6*His 标签。VEGF-CC 蛋白, Mouse/Rat (HEK293, His) 全长 116 个氨基酸,分子量为 14-23 kDa。

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描述

VEGF-CC protein stimulates cell proliferation, migration, and vascular permeability, which are critical for angiogenesis and endothelial cell dynamics. It plays a crucial role in the development of the venous and lymphatic vasculature during embryogenesis and in the maintenance of adult lymphatic endothelium. VEGF-CC Protein, Mouse/Rat (HEK293, His) is the recombinant mouse, rat-derived VEGF-CC protein, expressed by HEK293 , with C-6*His labeled tag. The total length of VEGF-CC Protein, Mouse/Rat (HEK293, His) is 116 a.a., with molecular weight of 14-23 kDa.

研究背景

VEGF-CC 是一种在血管生成和内皮细胞动力学中至关重要的生长因子,对细胞增殖和迁移发挥刺激作用,同时也影响血管的通透性。它在血管生成中发挥着至关重要的作用,特别是在胚胎发生过程中静脉和淋巴管系统的发育中。此外,VEGF-CC 有助于维持成人分化的淋巴内皮。该蛋白结合并激活 KDR/VEGFR2 和 FLT4/VEGFR3 受体,协调血管发育和稳态的重要信号通路。在结构上,VEGF-CC 形成具有非共价和反向平行排列的同二聚体。它与 FLT4/VEGFR3 的相互作用对于 FLT4/VEGFR3 同二聚化和随后的激活至关重要,突出了其在血管过程中的复杂调节作用。

生物活性

1. Immobilized mouse/rat VEGFC-His at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc, The EC50 of mouse VEGFR3-Fc is 17.4-40.6 ng/mL.
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is typically 0.1-2.612 μg/mL, corresponding to a specific activity of ≥ 382.85 units/mg.

  • Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 this effect is 1.04 μg/mL, corresponding to a specific activity is 961.54 units/mg.
种属

Mouse; Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

P97953/NP_033532.1/O35757 (A108-R223)

基因 ID

22341  [NCBI]/22341  [NCBI]/114111

蛋白结构
N-term
VEGF-CC (A108-R223)
Accession # P97953/NP_033532.1/O35757
6*His
C-term
同用名
Flt4-L; vascular endothelial growth factor C; VEGFC; VRP
氨基酸序列

AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR

分子量

14-23 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VEGF-CC Protein, Mouse/Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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VEGF-CC Protein, Mouse/Rat (HEK293, His)
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