1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-D
  6. VEGF-DD 蛋白, Rat (HEK293, Fc)

VEGF-DD 蛋白, Rat (HEK293, Fc)

目录号: HY-P73475
COA 产品使用指南

VEGF-DD 是一种生长因子,可刺激增殖、迁移和血管通透性。它对于血管发育和淋巴管内皮的维持至关重要。VEGF-DD 结合并激活 VEGFR-3,启动重要的信号传导通路。在结构上,它形成非共价同二聚体,在血管过程中发挥复杂的作用。VEGF-DD 蛋白, Rat (HEK293, Fc) 是重组的 VEGF-DD 蛋白,由 HEK293 表达,带有 N-hFc 标签。VEGF-DD 蛋白, Rat (HEK293, Fc) 全长 117 个氨基酸,分子量约为 ~50 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥360 In-stock
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg ¥4800 In-stock
500 μg ¥12000 In-stock
1 mg ¥18000 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

VEGF-DD, a growth factor, stimulates proliferation, migration, and vessel permeability. It is crucial for vascular development and maintenance of lymphatic endothelium. VEGF-DD binds and activates VEGFR-3, initiating essential signaling pathways. Structurally, it forms a non-covalent homodimer, playing an intricate role in vascular processes. VEGF-DD Protein, Rat (HEK293, Fc) is the recombinant rat-derived VEGF-DD protein, expressed by HEK293 , with N-hFc labeled tag. The total length of VEGF-DD Protein, Rat (HEK293, Fc) is 117 a.a., with molecular weight of ~50 kDa.

研究背景

VEGF-DD 是一种在血管生成、淋巴管生成和内皮细胞动力学中发挥关键作用的生长因子,具有刺激细胞增殖和迁移以及影响血管通透性的能力。其重要性延伸到胚胎发生过程中静脉和淋巴管系统的形成,以及成人分化淋巴管内皮的维持。在功能上,VEGF-DD 结合并激活 VEGFR-3 (Flt4) 受体,启动血管发育和稳态的关键信号通路。在结构上,VEGF-DD 作为同二聚体存在,具有非共价和反平行构型,强调了其在协调复杂血管过程中的复杂作用。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized recombinant Rat VEGF-DD at 10 μg/mL (100 μL/well) can bind biotinylated Mouse VEGFR-3. The ED50 for this effect is 312.9 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized recombinant Rat VEGF-DD at 10 μg/mL (100 μL/well) can bind biotinylated Mouse VEGFR-3 .The ED50 for this effect is 312.9 ng/mL.
种属

Rat

表达系统

HEK293

标签

N-hFc

蛋白编号

O35251 (F94-R210)

基因 ID

360457  [NCBI]

蛋白结构
N-term
hFc
VEGF-DD (F94-R210)
Accession # O35251
C-term
同用名
Vascular endothelial growth factor D; VEGF-D; FIGF
氨基酸序列

FAATFYDTETLKVIDEEWQRTQCSPRETCVEVASELGKTTNTFFKPPCVNVFRCGGCCNEESVMCMNTSTSYISKQLFEISVPLTSVPELVPVKIANHTGCKCLPTGPRHPYSIIRR

分子量

Approximately 50 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VEGF-DD Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
VEGF-DD Protein, Rat (HEK293, Fc)
目录号:
HY-P73475
需求量: