VEGF121 蛋白, Human (HEK293, N-His)

Vascular Endothelial Growth Factor A (VEGF-A) is a key member of the VEGF family of cytokines that can promote neovascularization and increase vascular permeability. VEGF121 Protein, Human (HEK293, N-His) is the recombinant human-derived VEGF121 protein, expressed by HEK293, with N-His labeled tag.

VEGF-A 是血管内皮生长因子 (VEGF) 家族的重要成员，其他成员包括 VEGF-B、-C、-D 和 PGF。VEGF-A 参与血管生成、血管发生和内皮细胞生长，诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡并诱导血管通透。VEGF-A 与 FLT1/VEGFR1 和 KDR/VEGFR2 受体、硫酸乙酰肝素和肝素结合。VEGF-A 还与 NRP1 结合，启动运动神经元轴突引导和细胞体迁移所需的信号通路，包括胚胎发育过程中面部运动神经元从菱形 4 到菱形 6 的尾部迁移。VEGF165 可能由肿瘤细胞分泌，以高亲和力与 VEGFR-1 和 VEGFR-2 受体结合^{[1][2][3][4][5][6][7][8]}。

Human

HEK293

N-His

P15692-9 (A27-R147)

7422

Partial

Approximately 15 kDa & 18-20 kDa,based on SDS-PAGE under reducing conditions,33-38 kDa,based on SDS-PAGE under non-reduced conditions,due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.