VEGF121 蛋白, Human (HEK293)

VEGF121 Protein is a subtype of Vascular Endothelial Growth Factor A (VEGF-A). VEGF-A is a key member of the VEGF family of cytokines that can promote neovascularization and increase vascular permeability. VEGF121 Protein, Human (HEK293) is the recombinant human-derived VEGF121 protein, expressed by HEK293 , with tag free. The total length of VEGF121 Protein, Human (HEK293) is 147 a.a., with molecular weight of ~19.9 & 17.0 kDa, respectively.

VEGF121 是血管内皮生长因子 A (VEGF-A) 的一个亚型。VEGF-A 是一种在血管生成、血管形成和内皮细胞生长中活跃的生长因子。VEGF-A 诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡并诱导血管通透化。VEGF-A 与 FLT1/VEGFR1 和 KDR/VEGFR2 受体以及硫酸肝素和肝素结合。它还与 NRP1/神经纤毛蛋白-1 受体结合。当 VEGF-A 与 NRP1 结合时，它会启动运动神经元轴突引导和细胞体迁移所需的信号通路，包括面部运动神经元在胚胎发育过程中从菱脑节 4 向菱脑节 6 的尾部迁移。此外，VEGF-A 还与 DEAR/FBXW7-AS1 受体结合。

1.Immobilized human VEGF121 at 2μg/mL (100μL/well) can bind human VEGFR2-Fc and the EC₅₀ is 5-50 ng/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) and the ED₅₀ is typically 3-15 ng/mL.

Human

HEK293

Tag Free

P15692-9 (M1-R147)

7422  [NCBI]

Full Length of Isoform-9

Approximately 19.9&17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.