VEGFR-1 蛋白, Mouse (HEK293, His)

The VEGFR-1 protein is a key tyrosine protein kinase receptor. VEGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VEGFR-1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of VEGFR-1 Protein, Mouse (HEK293, His) is 737 a.a., with molecular weight of ~130 kDa.

VEGFR-1是一种酪氨酸蛋白激酶，作为VEGFA、VEGFB和PGF的关键细胞表面受体，在胚胎血管发育、血管生成调节、细胞存活、迁移、巨噬细胞功能、趋化性和癌细胞中发挥着不可或缺的作用入侵。它在出生后视网膜玻璃体血管的退化中起到正调节作用。虽然 VEGFR-1 可能通过抑制内皮细胞过度增殖而充当胚胎血管生成的负调节因子，但它还表现出细胞类型特异性的增殖、存活和成年期血管生成促进作用，尤其是对 PGF 的反应。它对 VEGFA 具有高亲和力，可通过异二聚体形成调节 KDR 信号传导。作为 VEGFA 信号传导的潜在负调节因子，它限制游离 VEGFA，阻碍其与 KDR 的结合。配体结合触发各种信号级联，包括 PLCG 激活，导致二酰甘油和肌醇 1,4,5-三磷酸的产生，以及蛋白激酶 C 激活。此外，VEGFR-1 介导 PIK3R1 的磷酸化，启动磷脂酰肌醇 3-激酶途径，并激活 MAPK1/ERK2、MAPK3/ERK1 和 AKT1 信号传导途径。它还磷酸化 SRC、YES1、PLCG 和潜在的 CBL，促进 AKT1 和 PTK2/FAK1 的磷酸化。

Mouse

HEK293

C-His;C-10*His

P35969/NP_034358.2 (Y23-E759)

14254  [NCBI]

YGSGSKLKVPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKRLSITPPSACGRDNRQFCSTLTLDTAQANHTGLYTCRYLPTSTSKKKKAESSIYIFVSDAGSPFIEMHTDIPKLVHMTEGRQLIIPCRVTSPNVTVTLKKFPFDTLTPDGQRITWDSRRGFIIANATYKEIGLLNCEATVNGHLYQTNYLTHRQTNTILDVQIRPPSPVRLLHGQTLVLNCTATTELNTRVQMSWNYPGKATKRASIRQRIDRSHSHNNVFHSVLKINNVESRDKGLYTCRVKSGSSFQSFNTSVHVYEKGFISVKHRKQPVQETTAGRRSYRLSMKVKAFPSPEIVWLKDGSPATLKSARYLVHGYSLIIKDVTTEDAGDYTILLGIKQSRLFKNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPRPTITWLWHPCHHNHSKERYDFCTENEESFILDPSSNLGNRIESISQRMTVIEGTNKTVSTLVVADSQTPGIYSCRAFNKIGTVERNIKFYVTDVPNGFHVSLEKMPAEGEDLKLSCVVNKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEDILRKTEVLVRDSEAPHLLQNLSDYEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGAVESAAYLTVQGTSDKSNLE

Approximately 130 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.