VEGFR-3/FLT4 蛋白, Mouse (HEK293, hFc)

VEGFR-3/FLT4 proteins are tyrosine protein kinase receptors for VEGFC and VEGFD and are critical in adult lymphangiogenesis and embryonic vascular development. It promotes endothelial cell function, angiogenesis, and forms a positive feedback loop that increases VEGFC production. VEGFR-3/FLT4 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived VEGFR-3/FLT4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VEGFR-3/FLT4 Protein, Mouse (HEK293, hFc) is 751 a.a., with molecular weight of 115-160 kDa.

VEGFR-3/FLT4 蛋白是一种酪氨酸蛋白激酶，作为 VEGFC 和 VEGFD 的细胞表面受体发挥作用，在成人淋巴管生成中发挥关键作用，并在胚胎发育过程中对血管网络和心血管系统的发育做出重大贡献。其多种功能包括促进内皮细胞的增殖、存活和迁移，以及调节血管生成。FLT4 的激活会导致 VEGFC 的产生增加，并在较小程度上增加 VEGFA 的产生，从而建立增强 FLT4 信号传导的正反馈环。此外，VEGFR-3/FLT4 通过异二聚体的形成调节 KDR 信号传导。其信号级联涉及 MAPK1/ERK2、MAPK3/ERK1、MAPK8 和 JUN 通路以及 AKT1 通路的激活。VEGFR-3/FLT4 磷酸化 SHC1 并介导 PIK3R1（磷脂酰肌醇 3-激酶的调节亚基）的磷酸化。此外，它还能促进 MAPK8 在“Thr-183”和“Tyr-185”处的磷酸化，以及 AKT1 在“Ser-473”处的磷酸化，强调了其在协调对淋巴管生成和血管发育至关重要的复杂信号传导事件中的不可或缺的作用。

Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-hFc

P35917 (Y25-E775)

14257  [NCBI]

YSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEARPYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTAASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPTQLLRDALYLQCETTWGDQNFLSNLFVVHITGNELYDIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVPERRSQQTHTELSSILTIHNVSQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISVEWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSRQTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEFVEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDERLIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPGARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSME

115-160 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.