Vinculin 蛋白, Mouse (HEK293, His)

Vinculin Protein, a crucial actin filament (F-actin)-binding protein, actively engages in cell-matrix and cell-cell adhesion, regulating E-cadherin expression and enhancing mechanosensing with the E-cadherin complex for cell morphology and locomotion (By similarity). Vinculin self-associates and is part of a complex with THSD1, PTK2/FAK1, TLN1, and VCL (By similarity). Interactions with proteins like APBB1IP, NRAP, TLN1, SYNM, CTNNB1, SORBS1, and CTNNA1 serve specific cellular functions. The CTNNB1 interaction is crucial for vinculin's localization to cell-cell junctions and its role in regulating E-cadherin surface expression (By similarity). Additionally, vinculin binds to ACTN4, inducing conformational changes for functional versatility (By similarity). Vinculin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vinculin protein, expressed by HEK293, with C-His labeled tag. The total length of Vinculin Protein, Mouse (HEK293, His) is 1066 a.a., with molecular weight of ~118 kDa.

纽蛋白是一种重要的肌动蛋白丝 (F-肌动蛋白) 结合蛋白，积极参与细胞-基质粘附和细胞-细胞粘附过程。它在细胞表面 E-钙粘蛋白的表达中发挥调节作用，并增强 E-钙粘蛋白复合物的机械感应，有助于细胞形态和运动（通过相似性）。值得注意的是，纽蛋白表现出自缔合特性，并且是包含 THSD1、PTK2/FAK1、TLN1 和 VCL（通过相似性）的复合体的一部分。其相互作用延伸至各种蛋白质，例如 APBB1IP、NRAP、TLN1、SYNM、CTNNB1、SORBS1 和 CTNNA1，每种相互作用都服务于特定的细胞功能。与 CTNNB1 的相互作用对于纽蛋白定位于细胞-细胞连接及其在调节 E-钙粘蛋白的细胞表面表达中的作用尤其重要（通过相似性）。此外，纽蛋白与 ACTN4 结合，引发构象变化，进一步增强其功能多样性（通过相似性）。

Measured by its binding ability in a functional ELISA .Immobilized Vinculin at 1 μg/ml can bind Anti-Vinculin antibody, the EC₅₀ of human Vinculin protein is 1.426 ng/mL, corresponding to a specific activity is 7.012×10⁵ units/mg.

Mouse

HEK293

C-His

Q64727 (M1-Q1066)

22330  [NCBI]

Approximately 118 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.