VISTA/B7-H5 蛋白, Rhesus Macaque (HEK293, His)

VISTA/B7-H5 protein is an immune regulatory receptor that can inhibit the activation of T cells and regulate immune responses as a ligand and receptor. VISTA/B7-H5 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived VISTA/B7-H5 protein, expressed by HEK293 , with C-His labeled tag. The total length of VISTA/B7-H5 Protein, Rhesus Macaque (HEK293, His) is 162 a.a., with molecular weight of ~19.6 kDa.

VISTA/B7-H5 蛋白是一种免疫调节受体，可以作为配体和受体调节免疫反应。VISTA/B7-H5 蛋白是一种 I 型跨膜蛋白是肿瘤免疫治疗中检测肿瘤和免疫细胞的免疫调节蛋白^[1]。
VISTA/B7-H5 Protein 抑制T细胞活化，诱导 Foxp3 表达，并在肿瘤微环境中高度表达，阻断 VISTA/B7-H5 Protein 的表达可以增强小鼠的抗肿瘤免疫反应^[2]。

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in Jurkat human T lymphocytes.The ED₅₀ for this effect is 1.828 μg/mL in the presence of 5 μg/mL anti-CD3, corresponding to a specific activity is 547.046 U/mg.

Rhesus Macaque

HEK293

C-His

F7F6S6 (F33-A194)

709595  [NCBI]

FKVATLYSLYVCPEGQNVTLTCRFFGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSSCVAYPSSSQESENITAA

Approximately 32-45 kDa due to glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.