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Vitamin D-binding protein/GC 蛋白, Mouse (His)

目录号: HY-P72292
COA 产品使用指南

维生素 D 结合蛋白(GC 蛋白)在维生素 D 运输和储存以及细胞外 G 肌动蛋白的清除中发挥着关键作用。它增强中性粒细胞的 C5 α 趋化活性,参与巨噬细胞活化,并与 B 淋巴细胞上的膜结合免疫球蛋白结合。Vitamin D-binding protein/GC 蛋白, Mouse (His) 是重组的 维生素 D 结合蛋白/GC 蛋白,由 E. coli 表达,带有 N-His 标签。Vitamin D-binding protein/GC 蛋白, Mouse (His) 全长 460 个氨基酸,分子量约为 ~57.4 kDa。

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描述

Vitamin D-binding proteins (GC proteins) play key roles in vitamin D transport and storage and in the clearance of extracellular G-actin. It enhances the C5 alpha chemotactic activity of neutrophils, participates in macrophage activation, and binds to membrane-bound immunoglobulins on B lymphocytes. Vitamin D-binding protein/GC Protein, Mouse (His) is the recombinant mouse-derived Vitamin D-binding protein/GC protein, expressed by E. coli , with N-His labeled tag. The total length of Vitamin D-binding protein/GC Protein, Mouse (His) is 460 a.a., with molecular weight of ~57.4 kDa.

研究背景

维生素 D 结合蛋白(GC 蛋白)在维生素 D 运输和储存中发挥着关键作用,有助于细胞外 G 肌动蛋白的清除。此外,它还能增强炎症期间 C5 α 对中性粒细胞的趋化活性,并参与巨噬细胞激活。值得注意的是,GC 蛋白与 B 淋巴细胞表面的膜结合免疫球蛋白结合,并与 T 淋巴细胞膜上的 IgG Fc 受体相互作用,表明其参与免疫相关过程。此外,它与 LRP2 的相互作用对于肾脏摄取 GC 与 25-羟基维生素 D3 的复合物至关重要,突出了它在维生素 D 代谢中的重要性。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

P21614 (L17-S476)

基因 ID

14473  [NCBI]

蛋白结构
N-term
His
GC (L17-S476)
Accession # P21614
C-term
同用名
Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component
氨基酸序列

LERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQVNQLVKEVVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEGLERKLCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSNYGQAPLPLLVAYTKNYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMSNRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCCESTSEDCMASELPEHTIKICQNLSKKNSKFEECCQENTPMNIFMCTYFMPAAEPLQLPAIKLPTGKDLCGQSTTQAMDQYTFELSRRTQVPEVFLSKVLEPTLKTLRECCDTQDSVACFSTQSPLLKRQLTSFIEKGQEMCADYSENTFTEYKKKLAERLRTKTPNTSPAELKDMVEKHSDFASKCCSINSPPLYCSSQIDAEMIDTLQS

分子量

Approximately 57.4kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Vitamin D-binding protein/GC Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Vitamin D-binding protein/GC Protein, Mouse (His)
目录号:
HY-P72292
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