Vitamin D-binding protein/GC 蛋白, Human (HEK293, His)

GC protein is a multifunctional protein that promotes vitamin D transport and storage while scavenging extracellular G-actin to maintain cellular homeostasis. It enhances the chemotactic activity of C5 alpha toward neutrophils during inflammation, helps macrophage activation, and interacts with B lymphocyte and T lymphocyte membranes, indicating its involvement in the immune process. Vitamin D-binding protein/GC Protein, Human (HEK293, His) is the recombinant human-derived Vitamin D-binding protein/GC protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Vitamin D-binding protein/GC Protein, Human (HEK293, His) is 458 a.a., with molecular weight of ~53.0 kDa.

维生素D结合蛋白（GC蛋白）是一种参与多种生理过程的多功能蛋白质。它在维生素 D 的运输和储存中发挥着关键作用，有助于提高其全身可用性。此外，GC 蛋白可作为细胞外 G 肌动蛋白的清除剂，这是维持细胞稳态的关键功能。在炎症情况下，它增强 C5 α 对中性粒细胞的趋化活性，积极参与免疫反应。此外，GC 蛋白与巨噬细胞激活有关，有助于协调免疫过程。除了其免疫相关功能外，GC 蛋白还与 B 淋巴细胞上的膜结合免疫球蛋白结合，并与 T 淋巴细胞膜上的 IgG Fc 受体相互作用，表明它参与免疫细胞相互作用。值得注意的是，与 LRP2 的相互作用对于肾脏摄取 GC 与 25-羟基维生素 D3 的复合物至关重要，这凸显了其在维生素 D 代谢和体内平衡中的重要性。

Human

HEK293

C-6*His

P02774 (L17-L474)

2638  [NCBI]

LERGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPTELAKLVNKHSDFASNCCSINSPPLYCDSEIDAELKNIL

Approximately 53.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.