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  3. VNN1/Vanin-1 蛋白, Mouse (HEK293, Fc)

VNN1/Vanin-1 蛋白, Mouse (HEK293, Fc)

目录号: HY-P76126
COA 产品使用指南

Vanin-1 (VNN1) 是一种酰胺水解酶,可特异性水解 D-泛硫氨酸中的一个碳酰胺键。这种酶的功能对于回收泛酸(维生素 B5)、释放半胱胺至关重要。VNN1 分解 D-泛酸有助于维持足够的泛酸水平,这对于各种生化途径至关重要。这种酶作用强调了 VNN1 在调节维生素 B5 稳态和半胱胺释放中的作用,强调了它在细胞代谢中的重要性。VNN1/Vanin-1 蛋白, Mouse (HEK293, Fc) 是重组的 VNN1/Vanin-1 蛋白,由 HEK293 表达,带有 C-hFc 标签。VNN1/Vanin-1 蛋白, Mouse (HEK293, Fc) 全长 487 个氨基酸,分子量为 85-90 kDa。

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规格 价格 是否有货 数量
10 μg ¥1150 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

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描述

Vanin-1 (VNN1), an amidohydrolase, specifically hydrolyzes one carboamide linkage in D-pantetheine. This enzymatic function is crucial for recycling pantothenic acid (vitamin B5), releasing cysteamine. VNN1's breakdown of D-pantetheine contributes to maintaining sufficient pantothenic acid levels, vital for various biochemical pathways. This enzymatic action emphasizes VNN1's role in regulating vitamin B5 homeostasis and cysteamine release, highlighting its significance in cellular metabolism. VNN1/Vanin-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived VNN1/Vanin-1 protein, expressed by HEK293, with C-hFc labeled tag. The total length of VNN1/Vanin-1 Protein, Mouse (HEK293, Fc) is 487 a.a., with molecular weight of 85-90 kDa.

研究背景

Vanin-1 (VNN1) 是一种酰胺水解酶,具有水解 D-泛硫氨酸中的一个碳酰胺键的特定功能。这种酶活性在泛酸(维生素 B5)的回收中发挥着至关重要的作用,最终释放出半胱胺。通过促进 D-泛酸的分解,VNN1 有助于维持足够水平的泛酸的代谢过程,泛酸是各种生化途径的重要组成部分。这种酶作用强调了 VNN1 在调节维生素 B5 稳态和半胱胺释放中的重要性,突出了其在细胞代谢中的作用。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9Z0K8 (M1-S487)

基因 ID

22361  [NCBI]

蛋白结构
N-term
VNN1 (M1-S487)
Accession # Q9Z0K8
hFc
C-term
同用名
Pantetheinase; Pantetheine hydrolase; Vascular non-inflammatory molecule 1; Vanin-1
分子量

85-90 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

VNN1/Vanin-1 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VNN1/Vanin-1 Protein, Mouse (HEK293, Fc)
目录号:
HY-P76126
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