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  3. Transferases (EC 2)
  4. WEE2 蛋白, Human (Sf9, His, GST)

WEE2 蛋白, Human (Sf9, His, GST)

目录号: HY-P701729
产品使用指南

WEE2 蛋白是一种卵母细胞特异性酪氨酸激酶,在减数分裂前期 I 和中期 II 中发挥重要作用。它通过在“Tyr-15”处磷酸化 CDK1、抑制 CDK1 活性并防止折返来维持生发囊泡 (GV) 阶段的减数分裂停滞。WEE2 蛋白, Human (Sf9, His, GST) 是重组的 WEE2 蛋白,由 Sf9 insect cells 表达,带有 N-8*His, N-GST 标签。WEE2 蛋白, Human (Sf9, His, GST) 全长 566 个氨基酸。

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1.  客户无需承担相应的运输费用。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (Sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of WEE2 Protein, Human (Sf9, His, GST) is 566 a.a., .

研究背景

WEE2 蛋白是一种卵母细胞特异性蛋白酪氨酸激酶,在 I 前期和 II 中期的减数分裂调节中发挥着关键作用。其重要功能是在生发囊泡 (GV) 阶段维持卵母细胞减数分裂停滞,即在 dictyate 前期 I 的长时间静止期。WEE2 通过在“Tyr-15”处磷酸化 CDK1 来实现这一目标,从而抑制 CDK1 活性,并抑制 CDK1 的活性。防止减数分裂再进入。此外,WEE2 通过在“Tyr-15”处磷酸化 CDK1,有助于促进卵子激活过程中中期 II 的退出。这一作用确保了卵母细胞减数分裂的有序退出,促进原核形成并有助于复杂的生殖过程。

种属

Human

表达系统

Sf9 insect cells

标签

N-8*His;N-GST

蛋白编号

P0C1S8 (D2-H567)

基因 ID

494551

蛋白结构
N-term
8*His-GST
WEE2 (D2-H567)
Accession # P0C1S8
C-term
同用名
WEE2; Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

WEE2 Protein, Human (Sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
WEE2 Protein, Human (Sf9, His, GST)
目录号:
HY-P701729
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