XPNPEP3 蛋白, Human (His)

The XPNPEP3 protein catalyzes the removal of prolyl residues from N-terminal peptides (such as Leu-Pro-Ala) and has low activity towards Ala or Ser at the P1 site. XPNPEP3 Protein, Human (His) is the recombinant human-derived XPNPEP3 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag. The total length of XPNPEP3 Protein, Human (His) is 507 a.a., with molecular weight of ~65.0 kDa.

XPNPEP3 蛋白通过催化去除肽 N 末端倒数第二个脯氨酰残基（例如 Leu-Pro-Ala 等底物），在肽加工中发挥着至关重要的作用。此外，它对 P1 位点为 Ala 或 Ser 的肽表现出低活性。除了其肽酶功能之外，XPNPEP3 还表现出一个有趣的作用，因为它促进 TNFRSF1B 介导的 MAPK8/JNK1 和 MAPK9/JNK2 磷酸化，表明其具有作为 TNFRSF1B 衔接蛋白的潜在功能。值得注意的是，这种效应独立于 XPNPEP3 的肽酶活性，表明在细胞信号传导途径中具有双重作用。此外，XPNPEP3 可能在抑制 TNF-TNFRSF1B 信号传导诱导的细胞凋亡中发挥作用，强调其参与肽裂解以外的细胞过程。

Human

E. coli

N-6*His;C-6*His

Q9NQH7 (M1-S507)

63929  [NCBI]

MPWLLSAPKLVPAVANVRGLSGCMLCSQRRYSLQPVPERRIPNRYLGQPSPFTHPHLLRPGEVTPGLSQVEYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQDNNFLYLCGFQEPDSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALTGVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQQLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEAFLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQAELYEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVVTQDSPLILSADCPKEMNDIEQICSQAS

Approximately 65.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.