ygiS 蛋白, E.coli (His-SUMO)

The ygiS protein may be an important component of the deoxycholate transport system and play an important role in cellular responses to bile acid detergents, especially deoxycholate. Its absence leads to increased levels of intracellular deoxycholic acid, resulting in decreased cell growth. ygiS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived ygiS protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of ygiS Protein, E.coli (His-SUMO) is 515 a.a., with molecular weight of ~74.4 kDa.

ygiS 蛋白可能是脱氧胆酸盐转运系统的重要组成部分，在细胞对胆汁酸去污剂（尤其是脱氧胆酸盐）的反应中发挥着重要作用。在脱氧胆酸存在的情况下，其在 ygiS 缺失突变体中的表达导致细胞内脱氧胆酸水平升高，从而导致细胞生长下降。此外，在脱氧胆酸盐存在的情况下表达升高会导致细胞生长的完全抑制。ygiS 在响应胆汁酸去污剂（例如脱氧胆酸盐）方面的重要性，强调了它可能参与宿主针对胆囊和十二指肠细菌生长的防御机制。

E.coli

E. coli

N-His;N-SUMO

Q46863 (21A-535H)

947140  [NCBI]

ADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH

Approximately 74.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.