2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. YPR1 蛋白, Saccharomyces cerevisiae (His, Strep)

YPR1 蛋白, Saccharomyces cerevisiae (His, Strep)

目录号: HY-P701991
产品使用指南

在 SD 培养基的对数生长期，检测到 YPR1 蛋白的浓度为每个细胞 14100 个分子，表明在这些生长条件下其在细胞环境中的丰度。这些定量信息为了解 YPR1 的表达水平以及对数生长期间细胞过程中的潜在作用提供了宝贵的见解。YPR1 蛋白, Saccharomyces cerevisiae (His, Strep) 是重组的 YPR1 蛋白，由 E. coli 表达，带有 N-Strep, N-6*His 标签。YPR1 蛋白, Saccharomyces cerevisiae (His, Strep) 全长 311 个氨基酸。

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描述

During the log phase in SD medium, YPR1 protein is detected at a concentration of 14100 molecules per cell, indicating its abundance in the cellular environment under these growth conditions. This quantitative information offers valuable insights into YPR1's expression level and potential roles in cellular processes during logarithmic growth. YPR1 Protein, Saccharomyces cerevisiae (His, Strep) is the recombinant YPR1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of YPR1 Protein, Saccharomyces cerevisiae (His, Strep) is 311 a.a., .
研究背景

在 SD 培养基中的对数生长期，观察到 YPR1 蛋白以每个细胞 14100 个分子的浓度存在。这种定量见解强调了在这些特定生长条件下细胞环境中 YPR1 的丰度，提供了有关该蛋白表达水平以及对数生长过程中细胞过程中潜在作用的有价值的信息。
种属

Others
表达系统

E. coli
标签

N-Strep;N-6*His
蛋白编号

Q12458 (P2-Q312)
基因 ID

851974
蛋白结构
N-term
Strep-6*His
YPR1 (P2-Q312)
Accession # Q12458
C-term
同用名
YPR1; Putative reductase 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

YPR1 Protein, Saccharomyces cerevisiae (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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