YY1 蛋白, Human (His)

The multifunctional transcription factor YY1 controls a variety of cellular and viral genes by binding to sites that overlap with the transcription start site. YY1 recognizes the consensus sequence 5'-CCGCCATNTT-3' and exhibits context-dependent transcriptional regulation, with methylation of the initial CG dinucleotide affecting binding affinity. YY1 Protein, Human (His) is the recombinant human-derived YY1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of YY1 Protein, Human (His) is 101 a.a., with molecular weight of 17-20 kDa.

YY1 是一种多功能转录因子，通过与转录起始位点重叠的位点结合，对大量细胞和病毒基因发挥正向和负向控制。通过识别共有序列 5'-CCGCCATNTT-3'，YY1 可能会增强与某些具有较长基序的基因的结合，而初始 CG 二核苷酸的甲基化会显着降低结合亲和力。对转录调控的影响因 YY1 结合的环境而异，具有不同的机制，例如直接激活或抑制、通过辅因子募集进行间接调节，以及通过破坏结合位点或构象 DNA 变化来激活或抑制。YY1 的活性受到转录因子和细胞质蛋白的复杂调节，可以消除或完全抑制其介导的激活或抑制。值得注意的是，它在没有腺病毒 E1A 蛋白的情况下充当阻遏蛋白，但在腺病毒 E1A 蛋白存在的情况下转变为激活蛋白。YY1 与 SMAD1 和 SMAD4 合作，通过与 BMP 反应元件内的 SMAD 结合元件结合，参与骨形态发生蛋白 (BMP) 介导的心脏特异性基因表达。YY1 参与发育、分化，并建议招募 PRC2/EED-EZH2 复合物来靶向经历转录抑制的基因。此外，YY1 在 DNA 修复中发挥作用，表现出与 DNA 重组中间结构结合的倾向，并有助于增强子激活的调节。它被提议作为染色质重塑 INO80 复合物的核心成分，将该复合物靶向参与转录调控、DNA 复制和潜在 DNA 修复的 YY1 响应元件。

Human

E. coli

C-6*His

P25490 (V221-G321)

7528  [NCBI]

VTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHG

17-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.