Zika virus NS5 蛋白 (sf9, His)

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus NS5 protein (sf9, His) is the recombinant Virus-derived Zika virus NS5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Zika virus NS5 protein (sf9, His) is 652 a.a., with molecular weight of ~76.7 kDa.

Genome polyprotein 是一系列具有相似或不同功能的蛋白质单元，已被单细胞或多细胞生物体广泛利用，作为无数分子活动的浓缩器。Genome polyprotein 是共价连体的较小蛋白质链，在自然界中作为组织病毒（包括 HIV）蛋白质组的通用手段。Genome polyprotein 作为 NS4B 的信号肽，是 NS4B 抗干扰素活性所必需的。Genome polyprotein 通过干扰宿主 Dicer 抑制 RNA 沉默。Genome polyprotein 可能对病毒出芽起作用。通过 M 外结构域激活线粒体凋亡通路发挥细胞毒性作用。可能显示病毒蛋白活性^[1][2]。

Virus

Sf9 insect cells

C-His

ALU33341 (K2772-L3423)

/

Approximately 76.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,100 mM NaCl,50% Glycerol, pH 7.0, 0.5 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.