ZWINT/ZW10 interactor 蛋白, Human (GST)

ZWINT, a vital MIS12 complex component, is essential for kinetochore and spindle checkpoint activity. It plays a pivotal role in ZW10 targeting to the kinetochore in prometaphase, forming interactions with ZW10, MIS12, and NDC80 subunit of the NDC80 complex. ZWINT's engagement with KNL1, CETN3, DSN1, and PMF1 underscores its multifaceted role in orchestrating kinetochore complex assembly and functionality during mitosis. ZWINT/ZW10 interactor Protein, Human (GST) is the recombinant human-derived ZWINT/ZW10 interactor protein, expressed by E. coli , with N-GST labeled tag. The total length of ZWINT/ZW10 interactor Protein, Human (GST) is 277 a.a., with molecular weight of ~58.2 kDa.

ZWINT 作为 MIS12 复合体的关键组成部分，在着丝粒形成和纺锤体检查点活动中发挥着关键作用。它的参与对于 ZW10 在前中期正确靶向着丝粒至关重要。ZWINT 与综合体内的重要合作伙伴建立互动，包括 ZW10 和 MIS12。此外，ZWINT 在有丝分裂期间表现出与 NDC80 复合物的 NDC80 亚基的特定相互作用，有助于协调与纺锤体动力学相关的复杂细胞过程。除了这些相互作用之外，ZWINT 还与 KNL1、CETN3、DSN1 和 PMF1 相互作用，突出了其在促进着丝粒复合体的组装和功能方面的多方面作用。

Human

E. coli

N-GST

O95229 (1M-277P)

11130  [NCBI]

MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Approximately 58.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.