ZWINT/ZW10 interactor 蛋白, Human (His)

ZW10 interactor (ZWINT) is clearly involved in kinetochore function. ZWINT interacts with ZW10, a kinetochore protein, possibly regulating the association between ZW10 and kinetochores. ZWINT is required for kinetochore formation and spindle checkpoint activity and remains detectable on the kinetochore until late anaphase. ZWINT has a uniform distribution in the cytoplasm of interphase cells. ZWINT/ZW10 interactor Protein, Human (His) is the recombinant human-derived ZWINT/ZW10 interactor protein, expressed by E. coli , with N-6*His labeled tag. The total length of ZWINT/ZW10 interactor Protein, Human (His) is 277 a.a., with molecular weight of ~35 kDa.

ZW10 互作子 (ZWINT) 参与着丝粒的功能。ZWINT 与着丝点蛋白 ZW10 相互作用，可能调节着丝点与 ZW10 的结合。ZWINT 是 MIS12 复合体的一部分，是着丝点形成和纺锤体检查点活性所必需的。ZWINT 比 ZW10 更早定位到前期的着丝点，直到后期在着丝点上仍可检测到。ZWINT 在间期细胞的细胞质中分布均匀^[1][2][3][4]。

Human

E. coli

N-6*His

AAI10400.1 (M1-P277)

11130  [NCBI]

MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.