ABCG2 抗体 (YA4578)

ABCG2 Antibody (YA4578) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCG2.

ABCG2 是 ATP 结合盒（ATP-binding cassette，ABC）家族的一种具有广泛底物特异性的 ATP 依赖型转运蛋白，能主动将多种生理化合物、膳食毒素和外源性物质从细胞中排出；参与卟啉稳态，介导原卟啉 IX（protoporphyrin IX，PPIX）从线粒体到细胞质以及从细胞质到细胞外空间的转运，还参与细胞内血红素（heme）的输出；介导鞘氨醇-1-磷酸（sphingosine-1-P）的细胞外排；作为尿酸转运蛋白，参与肾脏和肾外的尿酸排泄；在肾脏中，还作为尿毒症毒素硫酸吲哚酚的生理性转运蛋白（By similarity）；参与类固醇（如雌酮 3-硫酸盐（estrone 3-sulfate，E1S）、3β-磺氧基-雄甾-5-烯-17-酮（3beta-sulfooxy-androst-5-en-17-one，DHEAS））和其他硫酸结合物的排泄；介导核黄素（riboflavin）和生物素（biotin）等维生素分泌到乳汁中（By similarity）；将脱镁叶绿酸 a（pheophorbide a）——一种具有光毒性的叶绿素卟啉分解代谢物排出，降低其生物利用度（By similarity）；在将外源性物质排除在大脑之外方面发挥重要作用（可能）；通过控制米托蒽醌、脱镁叶绿酸、喜树碱、甲氨蝶呤、叠氮胸苷以及蒽环类药物柔红霉素和阿霉素等多种药物和其他外源性物质的外排，使细胞对这些物质产生耐药性；在胎盘中，限制药物从母体血浆渗透到胎儿体内（By similarity）；可能通过阻断分化在早期干细胞自我更新中发挥作用（By similarity）；在炎性巨噬细胞中，将衣康酸（itaconate）从细胞质转运到细胞外，并限制参与抗菌先天免疫反应的 TFEB 依赖性溶酶体生物合成的激活。

Q9UNQ0

Predicted band size: 72 kDa; Observed band size: 50-70 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit, Monkey

WB: 1:500-1:2000; IHC: 1:100-1:500; IF: 1:50-1:500; FC: 1:200-1:400; ELISA: 1:10000

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ABCG2 Antibody(HY-P84881). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using ABCG2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P84881, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using ABCG2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P84881, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC, ELISA

液体

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ABCG2 aa 153-360.