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  4. ACADM 抗体 (YA1967)

ACADM 抗体 (YA1967)

目录号: HY-P82222
COA 抗体使用指南 技术支持

ACADM Antibody (YA1967) 是无偶联基团的、靶向 ACADM 的 IgG 抗体 (克隆号:YA1967),预测分子量为 47 kDa (实际观测:47 kDa)。ACADM Antibody (YA1967) 可用于人、 小鼠、 大鼠背景下的 WB、ICC/IF 实验。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

ACADM Antibody (YA1967) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1967), targeting ACADM, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). ACADM Antibody (YA1967) can be used for WB, ICC/IF experiment in human, mouse, rat background.

研究背景

ACADM(medium-chain specific acyl-CoA dehydrogenase)是线粒体脂肪酸 β-氧化(mitochondrial fatty acid beta-oxidation)第一步反应的关键酶之一,属于酰基辅酶 A 脱氢酶(acyl-CoA dehydrogenases)家族。它通过有氧催化将直链脂肪酰辅酶 A 硫酯(straight-chain fatty acyl-CoA thioester)的 C-2 和 C-3 位脱氢,生成反式-2-烯酰辅酶 A(trans-2-enoyl-CoA),同时将电子传递给电子转移黄素蛋白(electron transfer flavoprotein,ETF),最终通过 ETF-泛醌氧化还原酶(ETF-ubiquinone oxidoreductase)进入线粒体呼吸链。与其他酰基辅酶 A 脱氢酶不同,ACADM 特异性作用于饱和 6 至 12 碳长度的初级脂肪酰辅酶 A(by similar: PubMed:1970566,PubMed:21237683,PubMed:2251268,PubMed:8823175)。

基因 ID
蛋白数据库
中文名
ACADM 抗体 (YA1967)
同用名
ACADM; Medium-chain specific acyl-CoA dehydrogenase; mitochondrial; MCAD
分子量

Predicted band size: 47 kDa; Observed band size: 47 kDa

纯度

Affinity Purified

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Signal Transduction

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1/500-1/1000;IF: 1/50-1/200

  • Western blot analysis of extracts from K562 (lane 2(20μg)), A431 (lane 3(20μg)), Rat heart (lane 4(20μg)) and Mouse liver(lane 5(20μg)) using ACADM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
应用

WB, ICC/IF

性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthetic peptide of human ACADM

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
ACADM Antibody (YA1967)
目录号:
HY-P82222
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