ACTL6A 抗体 (YA2684)

ACTL6A Antibody (YA2684) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACTL6A.

ACTL6A 是一种染色质重塑因子（chromatin remodeling factor），通过 ATP 依赖的方式改变 DNA-核小体拓扑结构参与特定基因的转录激活与抑制。作为 SWI/SNF 染色质重塑复合物的核心组分，它能增强 SMARCA4/BRG1/BAF190A 的 ATP 酶活性，并促进该复合物与染色质/核基质的结合（by similar：在 NuA4 组蛋白乙酰转移酶复合物中参与 H4/H2A 乙酰化）。在神经发育过程中，ACTL6A 特异性存在于神经前体细胞 npBAF 复合物中，对神经干细胞的自我更新和增殖至关重要；当神经元退出细胞周期后，npBAF 中的 ACTL6A 会被神经元特异性亚基 ACTL6B 取代（by similar：nBAF 复合物与 CREST 协同调控树突生长相关基因）。此外，ACTL6A 可能也是染色质重塑 INO80 复合物的核心组分，参与转录调控、DNA 复制和修复。

O96019

Predicted band size: 47 kDa; Observed band size: 47 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105058

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis was performed on extracts from Ramos (lane 1, 20 μg), K562 (lane 2, 20 μg), HeLa (lane 3, 20 μg), Jurkat (lane 4, 20 μg), MCF-7 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using ACTL6A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human ACTL6A