Calponin 1 抗体 (YA3487)

Calponin 1 Antibody (YA3487) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Calponin 1.

Calponin 1 是一种与 thin filament 相关的蛋白质，参与调节和调控平滑肌收缩。它能够与 actin、calmodulin 和 tropomyosin 结合。Calponin 与 actin 的相互作用会抑制 actomyosin Mg-ATPase 活性（By similarity）。

P51911

Predicted band size: 33 kDa; Observed band size: 33 kDa

affinity purified

Non-conjugated

Unmodified

AB_3103205

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human, Mouse, Rat

Pig, Dog

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200;

• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using Calponin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using Calponin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Calponin.