Caspase-1 抗体

Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-1.

Caspase-1 是一种 thiol protease，通过蛋白水解切割其他蛋白质参与多种炎症过程。它能将炎症细胞因子 interleukin-1 beta (IL1B) 和 interleukin 18 (IL18) 的前体以及 pyroptosis 诱导剂 Gasdermin-D (GSDMD) 切割成活性成熟肽。作为炎症反应的启动子，Caspase-1 在细胞免疫中起关键作用：一旦通过 inflammasome 复合物的形成被激活，它通过切割 IL1B 和 IL18 启动促炎反应，释放参与多种炎症过程的成熟细胞因子。它还能在 Asp 残基的 P1 位点切割四肽。此外，Caspase-1 通过切割 GSDMD 启动 pyroptosis（一种程序性裂解性细胞死亡途径）。与 IL1B 的切割不同，GSDMD 的识别和切割并不严格依赖于共识切割位点，而是依赖于 Caspase-1 上的一个 exosite 界面，该界面识别并结合 Gasdermin-D 的 C 端部分 (GSDMD-CT)。在细菌感染时，Caspase-1 切割并激活 CASP7，促进质膜修复。在 inflammasome 激活期间，Caspase-1 在 DNA 病毒感染（而非 RNA 病毒感染）时通过切割 CGAS 来控制抗病毒免疫，使其失活。在凋亡细胞中，Caspase-1 切割 SPHK2，后者从细胞中释放并在细胞外保持酶活性（by similar）。

P29452

Predicted band size: 46 kDa; Observed band size: 46 kDa

affinity purified

Cytoplasm.

Non-conjugated

Unmodified

AB_3644161

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat,

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-F: 1:100-500; FC: 1μg/Test;ICC: 1:100-500; IF: 1:100-500

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  Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

液体

Supplied in 1% BSA, 0.01M PBS, 15mM Sodium Azide pH 7.4

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402