CD163 抗体

CD163 Antibody is an unconjugated, approximately 130 kDa, rabbit-derived, anti-CD163 polyclonal antibody. CD163 Antibody can be used for: WB, IHC-P, IHC-F, ICC/IF expriments in human, mouse, and predicted: rat, dog, pig, horse background without labeling.

CD163 是一种急性期调节受体，关键参与巨噬细胞对血红蛋白/触珠蛋白复合物的清除和内吞，从而保护组织免受游离血红蛋白引起的潜在氧化损伤。除了通过血红蛋白/结合珠蛋白的内吞作用在铁吸收和再循环中发挥作用之外，CD163 还以钙依赖性和 pH 依赖性方式与这些复合物结合。值得注意的是，与 HP*1S 表型的血红蛋白和二聚体触珠蛋白的复合物相比，它对 HP*1F 表型的血红蛋白和多聚体触珠蛋白的复合物表现出更高的亲和力。CD163 诱导一系列细胞内信号，涉及酪氨酸激酶依赖性钙动员、三磷酸肌醇产生以及 IL6 和 CSF1 的分泌。尤其是同工型 3，在细胞中表达时，显示出增强的配体内吞作用和表面表达。CD163 的脱落产生具有潜在抗炎特性的可溶形式 (sCD163)，可作为监测炎症条件下巨噬细胞活化的有价值的诊断参数。

Q86VB7

Predicted band size: 125 kDa; Observed band size: 150 kDa

affinity purified

Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.

Non-conjugated

Unmodified

AB_3103089

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse

Rat, Dog, Pig, Horse

WB: 1:500-2000; IHC-P: 1:100-500;IHC-F: 1:100-500; ICC/IF: 1:100-500

• 
  Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IHC-F, ICC/IF

液体

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% or 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human CD163: 1001-1121/1156