CEBP-alpha 抗体

CEBP-alpha Antibody is an unconjugated, approximately 39 kDa, rabbit-derived, anti-CEBP-alpha polyclonal antibody. CEBP-alpha Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, rat, and predicted: mouse, dog, pig, cow, rabbit, sheep, goat background without labeling.

C/EBP α 是一种转录因子，协调骨髓祖细胞、脂肪细胞、肝细胞以及肺和胎盘细胞的增殖停滞与分化。它直接结合 DNA 序列 5'-T[TG]NNGNAA[TG]-3'，作为不同靶基因的激活因子。在早期胚胎发生中，它与 CEBPB 具有重要且冗余的功能，对从共同髓系祖细胞 (CMP) 向粒细胞/单核细胞祖细胞 (GMP) 的转变至关重要，并对肝脏和肺的正常发育起关键作用 (By similarity)。此外，它对脂肪细胞的终末分化是必需的，并参与维持出生后的全身能量稳态和脂质储存 (By similarity)。C/EBP α 通过抑制 E2F1 下调维持细胞未分化和增殖状态的基因表达，这对诱导脂肪细胞和粒细胞终末分化至关重要。同时，E2F1 通过结合特定启动子并抑制 CEBPA 与其靶基因启动子的结合来阻断脂肪细胞分化。增殖停滞还依赖于其与 SWI/SNF 复合物的功能性结合。在肝脏中，它通过不同机制调节糖异生和脂肪生成：在糖异生中，它与 FOXO1 功能协同，结合 IRE 控制的启动子并调节 PCK1 或 G6PC1 等靶基因的表达；在脂肪生成中，它与 SREBF1 相互作用并转录协同激活 ACAS2 等特定脂肪生成靶基因的启动子。在脂肪组织中，它可能作为 FOXO1 的共激活因子，通过 FOXO1 结合位点作用于 ADIPOQ 启动子 (By similarity)。此外，C/EBP α 可作为显性负调控因子，结合 DNA 并具有转录激活活性，尽管效率远低于 isoform 2，且不抑制细胞增殖。它还能直接特异性增强核糖体 DNA 转录，通过与 RNA 聚合酶 I 特异性辅因子相互作用并诱导组蛋白乙酰化实现。

P49715

Predicted band size: 39kDa;Observed band size: 42 kDa

affinity purified

Nucleus

Non-conjugated

Unmodified

AB_3103079

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Rat

Mouse, Dog, Pig, Cow, Rabbit, Sheep, Goat

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1ug/Test;ICC: 1:100;IF: 1:100-500

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  Western blot analysis of extracts from M-Liver (lane 1) and HepG2 (lane 2) using CEBP-α antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

液体

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human CEBP-alpha: 251-358/358