Cleaved-Caspase 3 抗体 (YA4628)

Cleaved-Caspase 3 Antibody (YA4628) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

CASP3 是一种硫醇蛋白酶，作为主要的效应 caspase 参与细胞凋亡的执行阶段。在被起始 caspases（如 CASP8、CASP9 或 CASP10）切割并激活后，通过催化多种蛋白质的切割来介导细胞凋亡的执行。在细胞凋亡开始时，它会在 '216-Asp-|-Gly-217' 键处蛋白水解切割 poly(ADP-ribose) polymerase PARP1。类似功能包括切割和激活 sterol regulatory element binding proteins（SREBPs）在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间。此外，CASP3 还切割并激活 caspase-6（CASP6）、caspase-7（CASP7）和 caspase-9（CASP9），同时切割并失活 interleukin-18（IL18）。它还参与 huntingtin 的切割，并通过 RET 切割触发交感神经元中的细胞粘附。在氧化应激反应中，CASP3 切割并抑制 serine/threonine-protein kinase AKT1。在病毒诱导的细胞凋亡过程中，CASP3 通过切割抗病毒蛋白 CGAS、IRF3 和 MAVS 来抑制 I 型干扰素的产生，从而防止细胞因子过度产生。此外，CASP3 还参与 pyroptosis，通过切割和激活 gasdermin-E（GSDME）发挥作用。它还能切割 XRCC4 和 phospholipid scramblase 蛋白 XKR4、XKR8 和 XKR9，促进凋亡细胞表面的 phosphatidylserine 暴露。最后，在 DIABLO/SMAC 抑制 BIRC6-caspase 结合后，CASP3 切割 BIRC6。

P42574

Predicted band size: 32 kDa; Observed band size: 32, 17 kDa

affinity purified

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Western blot analysis was performed on extracts from Jurkat (1µM Staurosporine,12 hours) (lane 1, 15 μg) using Cleaved-Caspase 3 p17 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF

液体

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

A synthesized peptide derived from human active Caspase-3