Cripto1/CRIPTO 抗体 (YA2245)

Cripto1/CRIPTO Antibody (YA2245) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cripto1/CRIPTO.

Cripto 是一种 GPI 锚定的细胞膜蛋白，参与 Nodal 信号通路。细胞相关的 CRIPTO 作为 Nodal 的共受体在顺式 (cis) 中发挥作用。TMEM8A 介导的 CRIPTO 脱落通过允许可溶性 CRIPTO 在其他细胞上作为 Nodal 共受体来调节 Nodal 信号 (by similar: PubMed:27881714)。此外，它可能在决定随后产生中胚层的上胚层细胞中发挥作用 (by similar: PubMed:11909953)。

P13385

Predicted band size: 21 kDa; Observed band size: 21 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Stem Cells

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IF: 1:50-1:200; IP: 1:50; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from NCCIT (lane 1, 15 μg) using Cripto 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF, IP, FC

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Cripto1