Cas9 SP 抗体 (YA3558)

Cas9 SP Antibody (YA3558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cas9 SP.

Cas9 SP 是 CRISPR (clustered regularly interspaced short palindromic repeat) 系统中的一种关键蛋白，属于 II 型 CRISPR 系统。CRISPR 是一种适应性免疫系统，可保护宿主免受病毒、转座子和接合质粒等移动遗传元件的侵害。CRISPR 簇包含与入侵核酸互补的间隔序列 (spacer)，这些序列被转录并加工成 CRISPR RNA (crRNA)。在 II 型系统中，pre-crRNA 的正确加工需要 trans-encoded small RNA (tracrRNA)、内源性核糖核酸酶 3 (rnc) 和 Cas9 蛋白的参与。tracrRNA 引导核糖核酸酶 3 协助加工 pre-crRNA，而 Cas9 仅稳定 pre-crRNA:tracrRNA 相互作用，在 RNA 加工中无催化功能。随后，Cas9/crRNA/tracrRNA 复合物可内切酶活性切割与 spacer 互补的线性或环状 dsDNA 靶标，切割过程需要两种 guide RNAs (gRNA) 的存在。非互补链首先被内切酶切割，随后进行 3'-5' 外切酶修剪。DNA 结合和核酸酶活性均需要蛋白和两种 gRNAs。Cas9 通过识别 CRISPR 重复序列中的 protospacer adjacent motif (PAM) 来区分自身与非自身，因为细菌 CRISPR 位点内的靶标不具有 PAM。DNA 链分离和异源双链形成始于 PAM 位点，PAM 识别是催化活性所必需的。Cas9 SP 可赋予宿主对与 CRISPR spacer 序列同源的质粒的免疫力 (CRISPR 干扰)。by similar: CRISPR 系统广泛存在于细菌和古菌中，具有高度特异性，已成为基因编辑的重要工具。

Q99ZW2

Predicted band size: 158 kDa; Observed band size: 158 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Streptococcus pyogenes

WB: 1:500-1:1000; IHC: 1:100-1:200; IF: 1:50-1:200; FC: 1:50

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  Western blot analysis was performed on extracts from Cas9-10ng (lane 1, 10 ng), Cas9-5ng (lane 2, 5 ng), Cas9-2ng (lane 3, 2 ng), Cas9-1ng (lane 4, 1 ng), Cas9-0.5ng (lane 5, 0.5 ng), and Cas9-0.1ng (lane 6, 0.1 ng) using CRISPR-Cas9 SP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, FC

293T transfected with Cas9 cell lysates.

液体

Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

A synthesized peptide derived from human Cas9 SP