FGFR3 抗体 (YA438)

FGFR3 Antibody (YA438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR3.

FGFR3 是一种酪氨酸蛋白激酶 (tyrosine-protein kinase)，作为成纤维细胞生长因子 (fibroblast growth factors) 的细胞表面受体，在调控细胞增殖、分化和凋亡中发挥关键作用。它对软骨细胞 (chondrocyte) 的分化、增殖和凋亡调控至关重要，并参与正常骨骼发育。该蛋白同时调控成骨作用 (osteogenesis) 和成骨细胞 (osteoblasts) 的出生后骨矿化过程，既能促进软骨细胞凋亡，也可能促进癌细胞增殖。FGFR3 是内耳正常发育的必要因子，可磷酸化 PLCG1、CBL 和 FRS2。配体结合会激活多条信号通路：PLCG1 激活后产生二酰基甘油 (diacylglycerol) 和肌醇 1,4,5-三磷酸 (inositol 1,4,5-trisphosphate)；FRS2 磷酸化则介导 GRB2、GAB1、PIK3R1 和 SOS1 的募集，进而激活 RAS、MAPK1/ERK2、MAPK3/ERK1 和 MAP 激酶信号通路以及 AKT1 信号通路。该蛋白还参与维生素 D 代谢调控。导致激酶持续激活或影响 FGFR3 成熟、内化和降解的突变会引发异常信号传导。过度表达或持续激活的 FGFR3 会促进 PTPN11/SHP2、STAT1、STAT5A 和 STAT5B 的激活。其分泌型异构体 3 (isoform 3) 仍保留结合 FGF1 和 FGF2 的能力，可能干扰 FGF 信号传导。

Free

P22607

Predicted band size: 88 kDa;Observed band size: 95-130 kDa

Protein A affinity purified.

Cell membrane, Endoplasmic reticulum, Cytoplasmic vesicle, Secreted.

Non-conjugated

Unmodified

AB_3102484

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from HepG2 (lane 1) and M-lymph (lane 2) and K562 (lane 3) using FGFR3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FGFR3.AA range:30-63.