Gli1 抗体 (YA414)

Gli1 Antibody (YA414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli1.

Gli1 是一种转录激活因子，能够结合 DNA 共识序列 5'-GACCACCCA-3'，并在正常发育过程中调控特定基因的转录。它在颅面发育、指趾发育、中枢神经系统和胃肠道发育中发挥作用，并通过介导 SHH 信号通路参与细胞增殖和分化。此外，Gli1 还促进癌细胞迁移。by similar：与亚型 1 不同，Gli1 作为转录激活因子可激活 CD24 的表达，但激活的基因集合与亚型 1 不同。

Free

P08151

Predicted band size: 118 kDa; Observed band size: 150 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102317

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse, Rat

WB: 1:500-1:1,000 ;IHC-P:1:50-1:200

• 
  Western blot analysis of extracts from K-562(lane 2(20μg)，Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Gli1.AA range:38-82.