HSC70 抗体 (YA5396)

HSC70 Antibody (YA5396) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to HSC70.

HSC70 是一种分子伴侣，参与多种细胞过程，包括保护蛋白质组免受应激、新合成多肽的折叠和运输、伴侣介导的自噬 (chaperone-mediated autophagy)、错误折叠蛋白的激活降解、蛋白质复合物的形成和解离以及抗原呈递。它在蛋白质质量控制系统中发挥关键作用，确保蛋白质的正确折叠、错误折叠蛋白的重新折叠以及控制蛋白质的后续降解靶向。这一过程通过 ATP 结合、ATP 水解和 ADP 释放的循环实现，并由共伴侣 (co-chaperones) 介导。HSC70 对多肽的亲和力受其核苷酸结合状态调控：ATP 结合状态下对底物蛋白亲和力低，而 ATP 水解为 ADP 后构象变化会增加亲和力。其共伴侣分为三类：J 结构域共伴侣 HSP40s（刺激 HSC70 的 ATPase 活性）、核苷酸交换因子 (NEF) 如 BAG1/2/3（促进 ADP 到 ATP 的转换从而释放底物）以及 TPR 结构域伴侣如 HOPX 和 STUB1。此外，HSC70 在线粒体导入中起关键作用，将前蛋白递送至线粒体导入受体 TOMM70；抑制转录激活；作为 PRP19-CDC5L 复合物的组分参与剪接体激活；结合细菌脂多糖 (LPS) 介导炎症反应；在伴侣介导的自噬中识别含 -KFERQ 基序的底物并引导至溶酶体降解；与 LAMP2 协作促进胞质抗原的 MHC II 类呈递；参与 ER 相关降解 (ERAD) 通路；并通过与 DNAJC6 相互作用被招募至网格蛋白包被囊泡，激活 ATPase 活性以脱去网格蛋白涂层 (by similar)。

P11142

Observed band size: 73 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

IHC1:50-200; WB: 1:1000-2000

• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC-P, WB

液体

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthesized peptide derived from human HSC70