Hsp90 alpha/beta 抗体 (YA356)

Hsp90 alpha/beta Antibody (YA356) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp90 alpha/beta.

HSP90AA1 蛋白是一种分子伴侣，在参与细胞周期控制和信号转导的特定靶蛋白的成熟、结构维持和调节中发挥着至关重要的作用。HSP90AA1 通过与其伴侣功能至关重要的 ATP 酶活性相关的功能循环进行操作，诱导客户蛋白的构象变化，从而激活它们。与共伴侣的动态相互作用调节底物识别、ATP 酶循环和伴侣功能。它通过辅助伴侣与不同的客户蛋白类别结合，形成功能性伴侣，以 ADP 结合的部分开放构象释放正确折叠的客户蛋白和辅助伴侣。除了其伴侣活性外，HSP90AA1 通过将前蛋白递送至线粒体输入受体 TOMM70，在线粒体输入中发挥着关键作用。它还有助于在多个水平上调节转录机制，改变转录因子水平，调节表观遗传修饰剂，并参与基因启动子中的组蛋白驱逐。此外，HSP90AA1 结合细菌脂多糖，介导 LPS 诱导的炎症反应，包括 TNF 分泌。它拮抗 STUB1 介导的 TGF-β 信号传导抑制，并促进 TOMM70 与线粒体外膜中的 IRF3 或 TBK1 结合，从而促进宿主抗病毒反应。在微生物感染的情况下，HSP90AA1 干扰脑膜炎奈瑟氏球菌 NadA 介导的对人类细胞的侵袭，根据其表达水平影响表达 NadA 的大肠杆菌粘附和进入人类 Chang 细胞。

Free

P07900 / P08238

Predicted band size: 85 kDa; Observed band size: 90 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102170

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), Hela (lane 5, 15 μg), and K562 (lane 6, 15 μg) using Hsp90 alpha/beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Hsp90 beta.The exact sequence is proprietary to MCE.