JNK3 抗体 (YA3150)

JNK3 Antibody (YA3150) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JNK3.

The protein encoded by The related gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. The kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of The related gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for The related gene. A recent study provided evidence for translational readthrough in The related gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.

P53779

Predicted band size: 53 kDa; Observed band size: 53 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), PC-3 (lane 3, 15 μg), Hela (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and U2OS (lane 6, 15 μg) using JNK3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF, FC

液体

Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human JNK3