Notch1 抗体

Notch1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Notch1.

Notch1：该基因编码 NOTCH 蛋白质家族的成员。这种 I 型跨膜蛋白家族的成员具有共同的结构特征，包括由多个表皮生长因子样 (EGF) 重复序列组成的胞外结构域，以及由多个不同结构域类型组成的胞内结构域。Notch 信号通路是一种进化上保守的细胞间信号通路，它通过 Notch 家族受体与其同源配体的结合来调节物理相邻细胞之间的相互作用。编码的前原蛋白在反式高尔基体网络中进行蛋白水解处理，生成两条多肽链，这些多肽链异二聚化形成成熟的细胞表面受体。该受体在多种细胞和组织类型的发育中发挥作用。该基因的突变与主动脉瓣疾病、亚当斯-奥利弗综合征、T 细胞急性淋巴细胞白血病、慢性淋巴细胞白血病和头颈部鳞状细胞癌有关。[由 RefSeq 提供，2016 年 1 月]

Free

P46531

Predicted band size: 273 kDa; Observed band size: 125 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102971

Neuroscience

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Notch1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Notch1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling Notch1 Antibody (HY-P80768, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IHC-P, ICC/IF, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Notch1.The exact sequence is proprietary to MCE.