Phospho-PML Protein (Ser518) 抗体 (YA2632)

Phospho-PML Protein (Ser518) Antibody (YA2632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PML Protein (Ser518).

The protein encoded by The related gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The related gene is often involved in the translocation with the retinoic acid receptor alpha related gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of The related gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

P29590

Predicted band size: 98 kDa; Observed band size: 98,117 kDa

Affinity Purified

Non-conjugated

Phosphorylated

AB_3104979

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Western blot analysis of extracts from A549 (lane 2(20μg), A549 (lane 3(40μg), using Phospho-PML Protein (Ser518) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic phosphopeptide corresponding to residues surrounding Ser518 of human PML Protein