SUN1 抗体 (YA2022)

SUN1 Antibody (YA2022) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SUN1.

SUN1 是 LINC (LInker of Nucleoskeleton and Cytoskeleton) 复合体的组成部分，参与核纤层与细胞骨架之间的连接。LINC 复合体建立的核质相互作用在机械力穿过核膜的传递以及核移动和定位中起重要作用 (by similar)。SUN1 是 interkinetic nuclear migration (INM) 所必需的，并且对于大脑皮层中径向神经元迁移和胶质细胞迁移过程中的 nucleokinesis 和 centrosome-nucleus coupling 至关重要 (by similar)。它还参与减数分裂前期端粒与核膜的附着，涉及 SUN1/2:KASH5 LINC 复合体，其中 SUN1 和 SUN2 似乎至少部分冗余地发挥作用 (by similar)。SUN1 是配子发生所必需的，并参与配子发生所需的编码和非编码 RNA 的选择性基因表达 (by similar)。它有助于定义核孔复合体 (NPCs) 的分布 (by similar)，并且是 SYNE4 在核膜中有效定位所必需的 (by similar)。在精子发生过程中，SUN1 可能参与精子头部形成过程中的核重塑 (by similar)。此外，它可能在 DNA 修复中发挥作用，通过抑制非同源末端连接修复来促进 DNA 交联的修复。

O94901

Predicted band size: 87 kDa; Observed band size: 98 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104389

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), A431 (lane 2, 20 μg), and Ramos (lane 3, 20 μg) using SUN1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, FC

液体

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human SUN1