VPS35 抗体 (YA3314)

VPS35 Antibody (YA3314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VPS35.

VPS35 是 retromer cargo-selective complex (CSC) 的一个组成部分。CSC 被认为是 retromer 或其变体的核心功能组件，用于防止特定的跨膜 cargo 蛋白错误分选进入溶酶体降解途径。CSC 通过 RAB7A 和 SNX3 被招募到内体膜上。VPS35 主要通过与 cargo 蛋白的胞质结构域相互作用来介导 CSC 的 cargo 选择性，但这些相互作用亲和力较低，且 retromer SNX 蛋白也可能参与 cargo 的选择性，从而对 CSC 的经典功能提出了质疑。SNX-BAR retromer 介导 cargo 蛋白从内体到 trans-Golgi network (TGN) 的逆行运输，并参与内体到质膜的 cargo 蛋白回收过程。SNX3-retromer 介导 WLS 从内体到 TGN 的逆行运输，这一途径不同于 SNX-BAR retromer 通路。SNX27-retromer 被认为参与内体到质膜的 cargo 蛋白运输和回收，涉及广泛的 cargo 蛋白谱。CSC 似乎作为其他蛋白（如 WASH complex 和 TBC1D5）的招募中心。VPS35 是溶酶体酶受体 IGF2R 和 SLC11A2 的逆行运输所必需的，并参与调控 polymeric immunoglobulin receptor (pIgR-pIgA) 的 transcytosis。此外，VPS35 对 WASHC2C 和 TBC1D5 的内体定位至关重要，并通过 WASHC2 介导 CSC 与 WASH complex 的关联。在微生物感染中，异源三聚体 retromer CSC 介导人乳头瘤病毒从早期内体退出并递送至高尔基体。

Q96QK1

Predicted band size: 92 kDa; Observed band size: 81 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105677

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using VPS35 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using VPS35 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human VPS35