2. MCE Kits
  3. Cell Biology
  4. Cell Culture
  5. Sterile Antibiotic Solution
  6. Blasticidin S, Sterile

Blasticidin S, Sterile 

杀稻瘟菌素 S 无菌溶液

Cat. No.: HY-K1054
Manual SDS

MCE Blasticidin S, Sterile 来源于灰色链霉菌（Streptomyces griseochromogenes），是一种核苷类抗生素，主要通过干扰核糖体中肽键的形成而特异性抑制原核细胞或真核细胞的蛋白合成。

Blasticidin S, Sterile

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (100 μL)   Apply now  
1 mL ￥546 In-stock
5 mL ￥2184 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It can be used as a selection agent in both mammalian cells and bacterial cells.

Resistance to Blasticidin S is conferred by bsr and BSD. Therefore, Blasticidin S is a popular and effective antibiotic for selecting vectors bearing the bsr or BSD gene in mammalian cell lines. The recommended working concentration ranges from 1 to 20 μg/mL depending on the cell line and 50–100 μg/mL for bacterial selection. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S.

Storage

-20°C; 2 years.
Protocol

The Blasticidin S solution can be used according to the following methods:

1.   Add Blasticidin S solution in proportion to the sterile cell culture solution, mix well before use.

2.   Add Blasticidin S solution in proportion to the cell culture solution, filter and sterilize.

Components
Components HY-K1054-1 mL HY-K1054-5 mL
Blasticidin S, Sterile (10 mg/mL) 1 mL 5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
杀稻瘟菌素 S 无菌溶液
目录号：
HY-K1054
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