Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines

Aim: To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV Reverse Transcriptase on the growth of cultured normal and Cancer cells.

Methods: The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five Cancer cell lines have been tested. Cell viability was assessed by MTT assay.

Results: We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.

Conclusions: 2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other Telomerase inhibitors.