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  2. Dynein- and kinesin- mediated intracellular transport on microtubules facilitates RABV infection

Dynein- and kinesin- mediated intracellular transport on microtubules facilitates RABV infection

  • Vet Microbiol. 2021 Nov;262:109241. doi: 10.1016/j.vetmic.2021.109241.
Jing Xu 1 Jie Gao 1 Maolin Zhang 1 Danwei Zhang 1 Ming Duan 1 Zhenhong Guan 1 Yidi Guo 2
Affiliations

Affiliations

  • 1 Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, JilinUniversity, Changchun, Jilin, China.
  • 2 Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, JilinUniversity, Changchun, Jilin, China. Electronic address: guoyd@jlu.edu.cn.
Abstract

Rabies, caused by rabies virus (RABV), is one of the most important neurotropic zoonoses and poses a severe threat to human and animal health. Exploration of its mechanism of neural transmission is meaningful but still insufficient. Here, we described the effects of microtubule-depolymerizing drugs and inhibitors of microtubule motor proteins on RABV Infection. Colchicine, a microtubule-depolymerizing drug, significantly impeded RABV production in N2a cells. Overexpression of CC1 or p50 attenuated viral Infection through the functional disruption of cytoplasmic dynein, which was consistent with the inhibitory effect of Na3VO4, a dynein activity inhibitor. Moreover, transfection with Flag-KHCct impaired RABV Infection, as cytoplasmic kinesin-based motility was blocked. These results demonstrated that RABV can infect N2a cells in a manner that depends on microtubule integrity as well as dynein and Kinesin function.

Keywords

Dynein; Kinesin; Microtubules; RABV; Trafficking.

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