1. Academic Validation
  2. Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma

Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma

  • Cell Death Dis. 2021 Nov 9;12(11):1063. doi: 10.1038/s41419-021-04346-8.
Ce Li 1 Wenming Li 1 Shengda Cao 1 Jianing Xu 1 Ye Qian 1 Xinliang Pan 1 Dapeng Lei 1 Dongmin Wei 2
Affiliations

Affiliations

  • 1 Department of Otorhinolaryngology, Qilu Hospital, Shandong University; NHC Key Laboratory of Otorhinolaryngology (Shandong University), 107 West Wenhua Road, 250012, Jinan, Shandong, China.
  • 2 Department of Otorhinolaryngology, Qilu Hospital, Shandong University; NHC Key Laboratory of Otorhinolaryngology (Shandong University), 107 West Wenhua Road, 250012, Jinan, Shandong, China. entwdm@163.com.
Abstract

Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal Cancer, which is characterized by high early metastasis rate and poor prognosis. It is reported that circular RNA is involved in the occurrence and development of cancer; however, the role of circRNA in hypopharyngeal Cancer has little been investigated. We performed hypopharyngeal carcinoma circRNA microarray and qRT-PCR verification. The results showed circ_0058106 expression level was significantly upregulated in tumor tissues than in corresponding normal tissues. We found that circ_0058106 upregulation promoted proliferation, migration and invasion of HSCC cells, while knockdown of circ_0058106 inhibited proliferation, migration and invasion of HSCC cells both in vitro and in vivo. Bioinformatics predicted circ_0058106 may interact with miR-185-3p. We verified circ_0058106 directly bound miR-185-3p and downregulated miR-185-3p expression by using dual-luciferase reporter assay and qRT-PCR. Moreover, we proved circ_0058106 promoted HSCC cells tumorigenesis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway via miR-185-3p. In conclusion, our findings firstly confirmed the carcinogenic effect of circ_0058106 in promoting HSCC cells tumorigenesis, metastasis, invasion and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through sponging miR-185-3p, indicating that circ_0058106 may be a new therapeutic target and prognostic marker for HSCC.

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