2. Academic Validation
  3. Molecular structure of VEGFA polysaccharide protein and its regulation of monocyte infiltration and oxidative stress after myocardial infarction

Molecular structure of VEGFA polysaccharide protein and its regulation of monocyte infiltration and oxidative stress after myocardial infarction

  • Int J Biol Macromol. 2025 May;310(Pt 3):143199. doi: 10.1016/j.ijbiomac.2025.143199.
Zhenyu Huang 1,  Bohan Wen 2,  Ming Wang 3,  Yanqiao Lu 4,  Qingqi Ji 4,  Ju Mei 5,  Xin Shi 6,  Zhaolei Jiang 7
Affiliations

Affiliations

  • 1 Department of Cardiology, Shanghai Chest Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, China; Department of Central Laboratory, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.
  • 2 Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
  • 3 Department of Cardiology, Huadong Hospital, Fudan University, Shanghai, 200040, China.
  • 4 Department of Cardiology, Shanghai Chest Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, China.
  • 5 Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China. Electronic address: ju_mei63@126.com.
  • 6 Department of Cardiology, Shanghai Chest Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, China. Electronic address: iskysx@163.com.
  • 7 Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China. Electronic address: drjiangzhaolei@126.com.
PMID: 40258548 DOI: 10.1016/j.ijbiomac.2025.143199
Abstract

The pathogenesis of myocardial infarction (MI) is complex, involving multiple biomarkers and cell signaling pathways. The aim of this study was to elucidate the molecular structure of VEGFA dioglycan protein and explore how it regulates monocyte infiltration and oxidative stress response after myocardial infarction, so as to provide a new molecular target for the treatment of myocardial infarction. Differential expression analysis and enrichment analysis were performed to investigate the composition and characteristics of immune cells in myocardial infarction. The regulatory network was constructed by network analysis, and in vitro experiments were carried out by BMDM isolation culture. Animal experiments were conducted in mouse models, and data were verified and statistically analyzed by combining immunohistochemical staining, Real-Time PCR, Western blot and enzyme-linked immunosorbent assay (ELISA). Genome-wide association studies (GWAS) and single-cell data successfully identified key immune-related genes and analyzed differentially expressed mRNA and its characteristics in myocardial infarction. The immune microenvironment of myocardial infarction was investigated, the differentially expressed circRNA and miRNA were characterized, and the circrNa-mirNA-mrna regulatory network was constructed. The characteristics of differentially expressed proteins in myocardial infarction and the changes of mRNA during oxidative stress were identified and compared. By analyzing the changes in chromatin accessibility, the regulatory network between oxidative stress and myocardial infarction in immune cells was constructed, and the expression and co-localization of oxidative stress in myocardial infarction were verified.

Keywords

Molecular structure; Monocyte infiltration; Myocardial infarction; Oxidative stress; VEGFA diglycan protein.

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