2. MCE Kits
  3. Protein Biology
  4. Protein Sample Preparation
  5. Protease Inhibitor Cocktail
  6. Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 

蛋白酶抑制剂 Cocktail (EDTA-Free,100× in DMSO)

Cat. No.: HY-K0010
Manual COA SDS 技术支持

MCE 蛋白酶抑制剂 Cocktail (EDTA-Free, 100× in DMSO) 可以用于细胞或组织提取物用来增加蛋白稳定性。

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (100 μL)   Apply now  
1 mL ￥230 In-stock
10 mL ￥1630 In-stock
50 mL ￥6500 In-stock
100 mL ￥9750 In-stock

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的 1587 篇科研文献

WB
IP

    purchased from MCE. Usage Cited in: Mol Cancer. 2024 Apr 4;23(1):70.  [Abstract]

    Western blot analysis of EVs markers and endoplasmic reticulum marker in whole cell lysates and EVs.

    purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Nov;21(11):1251-1265.  [Abstract]

    The cels were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

    purchased from MCE. Usage Cited in: Neuro Oncol. 2024 Aug 2:noae147.  [Abstract]

    Co-immunoprecipitation of TNFR1 or VASN in T4121 and WF19 GSCs and immunoblotting for TNFR1 and VASN. IgG was used as the control antibody for IP.

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Mol Cancer. 2024 Apr 4;23(1):70.  [Abstract]

      Customer Validation
      Western blot analysis of EVs markers and endoplasmic reticulum marker in whole cell lysates and EVs.

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Aug 20.  [Abstract]

      Customer Validation
      The cels were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
      Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Neuro Oncol. 2024 Aug 2:noae147.  [Abstract]

      Customer Validation
      Co-immunoprecipitation of TNFR1 or VASN in T4121 and WF19 GSCs and immunoblotting for TNFR1 and VASN. IgG was used as the control antibody for IP.

      • 描述及优势

      • 价格比较

      • 储存条件 & 期限

      • 操作流程

      • 注意事项

      • 包装

      • 文件资料

      描述及优势

      在稳定环境下内源性蛋白的产生和降解通常处于动态平衡，在细胞水平上趋于稳定。而在体外研究中，蛋白的降解风险会增加，为防止蛋白降解，可以添加小分子抑制剂来阻断蛋白降解。

      MCE 蛋白酶抑制剂 Cocktail (EDTA-Free, 100× in DMSO) 由 6 种蛋白酶抑制剂混合制成，用于保护蛋白的完整性。本品可用于蛋白质免疫分析 (WB)、免疫沉淀 (IP)、免疫共沉淀 (Co-IP)、pull-down、免疫组织化学 (IHC)、免疫荧光 (IF)、激酶研究等多种分析。
      价格比较
      蛋白酶抑制剂 Cocktail (EDTA-Free,100× in DMSO) Price Comparison
      储存条件 & 期限

      -20°C, 2 年
      操作流程

      使用前需将本产品在室温下解冻，按照 1:100 (v/v) 的比例将该抑制剂混合物加入到裂解液中，混匀即可。
      注意事项

      1. 尽量避免反复冻融，以免效价下降。

      2. DMSO 在低温下解冻易结晶，可置于 25℃ 解冻。

      3. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。

      4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
      包装
      Cat. No. Product Name Package
      HY-K0010-1 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL
      HY-K0010-10 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 10
      HY-K0010-50 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 50
      HY-K0010-100 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 100
      文件资料

      Manual (593 KB) SDS (4582 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

      您最近查看的产品:

      Your information is safe with us. * Required Fields.

         产品名称：

      		  

      * 需求量：

      * 客户姓名：

      		 

      * Email：

      * 电话：

      		 

      * 公司或机构名称：

         留言给我们：

      Bulk Inquiry

      Inquiry Information

      产品名称：
      蛋白酶抑制剂 Cocktail (EDTA-Free,100× in DMSO)
      目录号：
      HY-K0010
      需求量：

      Request for COA Report

      Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

      .

         产品名称：

      		  

      * Lot #：

      * 客户姓名：

      		 

      * Email：

         电话：

      		 

      * 部门：

      * 公司或机构名称：

      		 

         留言给我们：

      Request for COA Report

      We have received your request and will respond to you as soon as possible.

      嗨！很高兴为您提供帮助！

      尊敬的 MCE 客户您好，
      请您选择所在区域，我们将转接对应客服为您服务！

      热门区域

      A

      B

      C

      F

      G

      H

      J

      L

      N

      Q

      S

      T

      X

      Y

      Z

      热 A B C F G H J L N Q S T X Y Z