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  5. Phosphatase Inhibitor Cocktail
  6. Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 

磷酸酶抑制剂 Cocktail II (100× in ddH2O)

Cat. No.: HY-K0022
Manual COA SDS 技术支持

磷酸酶抑制剂 Cocktail Ⅱ (100× in ddH2O) 由 5 种磷酸酶抑制剂混合制成，可以广谱的抑制酸性磷酸酶、碱性磷酸酶和蛋白酪氨酸磷酸酶（PTPs），用于维持蛋白的磷酸化状态。

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (100 μL)   Apply now  
1 mL ￥250 In-stock
10 mL ￥1720 In-stock
50 mL ￥7189 In-stock
100 mL ￥10790 In-stock

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的 411 篇科研文献

WB

    purchased from MCE. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11.  [Abstract]

    After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent e

    purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322.  [Abstract]

    Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

    purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Nov;21(11):1251-1265.  [Abstract]

    The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

    purchased from MCE. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764.  [Abstract]

    Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322.  [Abstract]

      Customer Validation
      Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11.  [Abstract]

      Customer Validation
      After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent e

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Aug 20.  [Abstract]

      Customer Validation
      The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
      Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Nat Neurosci. 2023 Mar 27.  [Abstract]

      Customer Validation
      Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

      • 描述及优势

      • 价格比较

      • 储存条件 & 期限

      • 操作流程

      • 包装

      • 文件资料

      描述及优势

      在稳定环境下内源性蛋白的产生和降解通常处于动态平衡，在细胞水平上趋于稳定。而在体外研究中，蛋白的降解风险会增加，为防止蛋白降解，可以添加小分子抑制剂来阻断蛋白降解。

      MCE 磷酸酶抑制剂 Cocktail II (100× in ddH2O) 由 5 种磷酸酶抑制剂混合制成，可以广谱的抑制酸性磷酸酶、碱性磷酸酶和蛋白酪氨酸磷酸酶 (PTPs)，用于维持蛋白的磷酸化状态。本品可用于蛋白质免疫分析 (WB)、免疫沉淀 (IP)、免疫共沉淀 (Co-IP)、pull-down、免疫组织化学 (IHC)、免疫荧光 (IF)、激酶研究等多种分析。
      价格比较
      磷酸酶抑制剂 Cocktail II (100× in ddH<sub>2</sub>O) Price Comparison
      储存条件 & 期限

      -20℃, 2 年
      操作流程

      使用前需将本产品在室温下解冻，按照 1:100 (v/v) 的比例将该抑制剂混合物加入到裂解液中，混匀即可。
      包装
      Cat. No. Product Name Package
      HY-K0022-1 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL
      HY-K0022-10 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 10
      HY-K0022-50 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 50
      HY-K0022-100 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 100
      文件资料

      Manual (636 KB) SDS (4575 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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