2. Academic Validation
  3. RD21 enhances resistance to the strawberry vein banding virus by promoting autophagy-mediated degradation of the viral silencing suppressor P6 protein

RD21 enhances resistance to the strawberry vein banding virus by promoting autophagy-mediated degradation of the viral silencing suppressor P6 protein

  • Plant Biotechnol J. 2025 Jun 8. doi: 10.1111/pbi.70168.
Xianchu Yang # 1,  Jiying Feng # 1,  Kai Xu # 1,  Jincheng Han 1,  Minghui Zhang 1,  Aiwen Cheng 1,  Zhanqi Wang 2,  Fang Wang 3,  Xueping Zhou 4,  Lei Jiang 1 5 6 7,  Tong Jiang 1 5 6 7
Affiliations

Affiliations

  • 1 School of Plant Protection, Anhui Agricultural University, Hefei, China.
  • 2 Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, China.
  • 3 Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
  • 4 State Key Laboratory for Biology of Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences, Beijing, China.
  • 5 Key Laboratory of Agri-Products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, Hefei, China.
  • 6 Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei, China.
  • 7 Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei, China.
  • # Contributed equally.
PMID: 40483578 DOI: 10.1111/pbi.70168
Abstract

Responsive to desiccation 21 (RD21) is a papain-like cysteine protease (PLCP). PLCPs are involved in plant growth and development, as well as stress and plant immune responses. Currently, few studies have reported on the involvement of PLCPs in defence against viral infections. Here, we show that RD21 of strawberry (Fragaria vesca) interacts with the strawberry vein banding virus (SVBV) P6 protein. SVBV Infection induces FvRD21 expression. Subcellular localization revealed that FvRD21 and P6 colocalize in the perinuclear and cytoplasmic regions. Further analysis showed that FvRD21 overexpression induces the Autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry Plants. Moreover, mutant FvRD21H300A lost its ability to interact with SVBV P6 and did not affect P6 accumulation and VSR function. Additionally, Abscisic acid (ABA) pretreatment of strawberry Plants promoted FvRD21 upregulation and inhibited SVBV Infection, and FvRD21 overexpression activated the ABA pathway. In summary, FvRD21 inhibits SVBV Infection by degrading SVBV P6 through the Autophagy pathway. Hence, this study provides a novel report on the defence strategy of strawberry Plants against SVBV infections.

Keywords

Fragaria vesca; autophagy; responsive to desiccation 21 (RD21); strawberry vein banding virus (SVBV) P6.

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