KIAA1429-mediated ZFPM2-AS1 m6A modification promotes the proliferation, migration and invasion of osteosarcoma cells

Osteosarcoma is the most prevalent malignant bone tumor in pediatric and adolescent populations. N⁶-methyladenosine (m⁶A) is a post-transcriptional modification of RNA, and the most prevalent internal chemical modification of mRNA. KIAA1429, also known as virus-like m⁶A methyltransferase-associated, is a key component of the m⁶A methyltransferase complex, and the largest protein within this complex. Long non-coding RNAs (lncRNAs) are a class of transcripts >200 nucleotides in length that are able to regulate gene expression. Friend of GATA family member 2-antisense 1 (ZFPM2-AS1) is a lncRNA that has been observed to exhibit aberrantly elevated expression in gastric Cancer. The present study aimed to explore the expression levels of KIAA1429 and ZFPM2-AS1 in osteosarcoma tissues and 143B and MG63 osteosarcoma cell lines. The results of bioinformatics analysis, reverse transcription-quantitative PCR (qPCR) and western blotting revealed that the expression of KIAA1429 and ZFPM2-AS1 in osteosarcoma tissues and cell lines was upregulated compared with that in the corresponding normal tissues or cells. The association between KIAA1429-mediated m⁶A modifications and ZFPM2-AS1 stability in osteosarcoma cells was confirmed using m⁶A-methylated RNA immunoprecipitation-qPCR and actinomycin D assays. In addition, in Cell Counting Kit-8 and Transwell assays KIAA1429 overexpression promoted the proliferation, invasion and migration of 143B and MG63 osteosarcoma cells, and these promotive effects were attenuated by ZFPM2-AS1 knockdown. These findings suggest a potential novel approach for molecular therapy in the treatment of osteosarcoma.

KIAA1429; ZFPM2-AS1; m6A; molecular mechanisms; osteosarcoma.