2. Academic Validation
  3. Activation of lands cycle-mediated inflammation in living macrophages exposed to label-free particulate matter

Activation of lands cycle-mediated inflammation in living macrophages exposed to label-free particulate matter

  • J Hazard Mater. 2025 Oct 1:499:140027. doi: 10.1016/j.jhazmat.2025.140027.
Jeongwon Park 1,  Jueun Lee 2,  Hyun-Jin Kim 3,  Su-Hyun Chae 4,  JinO Shin 1,  Jae-Hyuk Lee 5,  Yeongmi Cheon 6,  Youngae Jung 2,  Seul-Ki Mun 7,  Jong-Jin Kim 7,  Sung-Hak Kim 8,  Geum-Sook Hwang 9,  Seongsoo Lee 10
Affiliations

Affiliations

  • 1 Aging Research Group, Honam Regional Center, Korea Basic Science Institute, Gwangju 61751, South Korea; Department of Animal Science, Chonnam National University, Gwangju 61186, South Korea.
  • 2 Integrated Metabolomics Research Group, Metropolitan Seoul Center, Korea Basic Science Institute, Seoul 03760, South Korea.
  • 3 Department of Animal Science, Chonnam National University, Gwangju 61186, South Korea; Center for Aging Research, Department of Dermatology, Chobanian & Avedisian School of Medicine, Boston University, MA 02118, USA.
  • 4 Integrated Metabolomics Research Group, Metropolitan Seoul Center, Korea Basic Science Institute, Seoul 03760, South Korea; Department of Chemistry, Sungkyunkwan University, Suwon, Gyeonggi 16419, South Korea.
  • 5 Aging Research Group, Honam Regional Center, Korea Basic Science Institute, Gwangju 61751, South Korea; Department of Pathology, School of Medicine, Stanford University, CA 94305, USA.
  • 6 Aging Research Group, Honam Regional Center, Korea Basic Science Institute, Gwangju 61751, South Korea; Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, South Korea.
  • 7 Department of Biomedical Science, Sunchon National University, Suncheon, Jeonnam 57922, South Korea.
  • 8 Department of Animal Science, Chonnam National University, Gwangju 61186, South Korea. Electronic address: sunghakkim@chonnam.ac.kr.
  • 9 Integrated Metabolomics Research Group, Metropolitan Seoul Center, Korea Basic Science Institute, Seoul 03760, South Korea; College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea. Electronic address: gshwang@kbsi.re.kr.
  • 10 Aging Research Group, Honam Regional Center, Korea Basic Science Institute, Gwangju 61751, South Korea; Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi 17546, South Korea; Department of Life Science, Hanyang University, Seoul 04763, South Korea. Electronic address: soolee@kbsi.re.kr.
PMID: 41046674 DOI: 10.1016/j.jhazmat.2025.140027
Abstract

Particulate matter (PM) is a pollutant of great concern and has severe impacts on human health. Macrophages (Mφ) play a crucial role in the response to PM exposure, as they undergo metabolic reprogramming associated with inflammation. However, upon exposure to PM, the metabolic changes that occur in living Mφ remain unclear. Here, we present evidence that PM activates Lands cycle-mediated inflammatory mechanisms using 3D-holotomography (3D-HT) technique integrated with multi-omics analysis. We show that internalization of PM results in abnormal lipid droplet (LD) dynamics, potentially leading to changes in lipid metabolite levels. Additionally, our multi-omics data reveal that alterations in lipid metabolism mediated by the Lands cycle leads to the increased production of inflammatory lipid mediators, including lysophospholipids, ceramides and sphingomyelins. Furthermore, by performing lipid metabolic imaging in living Mφ, we elucidated changes in the activity of altered lipid metabolism and the spatiotemporal correlation between inflammation and LD dynamics. Our findings suggest that the developed approach can provide a new experimental strategy to identify lipid metabolism biomarkers in living cells upon PM exposure.

Keywords

3D-holotomography; Lands cycle; Macrophage; Multi-omics analysis; Particulate matter.

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