ACK1 蛋白, Human (sf9, GST)

The ACK1 protein is a non-receptor tyrosine protein and serine/threonine protein kinase that orchestrates cellular physiology by affecting cell spreading, migration, survival, growth, and proliferation. It phosphorylates AKT1, AR, MCF2, WASL and WWOX, affecting various cellular processes. ACK1 Protein, Human (sf9, GST) is the recombinant human-derived ACK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of ACK1 Protein, Human (sf9, GST) is 367 a.a., with molecular weight of ~62 kDa.

ACK1 蛋白是一种非受体酪氨酸蛋白和丝氨酸/苏氨酸蛋白激酶，在细胞生理学中发挥多方面的作用，通过将细胞外信号转导至胞质和核效应器来影响细胞的扩散、迁移、存活、生长和增殖。它磷酸化 AKT1、AR、MCF2、WASL 和 WWOX，影响各种细胞过程。ACK1 参与运输和网格蛋白介导的内吞作用，与表皮生长因子受体 (EGFR) 和网格蛋白结合，调节配体诱导的 EGFR 降解，并有助于 EGFR 在早期内体限制膜上的积累。作为 CDC42 的下游效应器，ACK1 通过 BCAR1 磷酸化介导 CDC42 依赖性细胞迁移。在大脑发育和成人突触功能中，ACK1可能发挥作用。ACK1 通过磷酸化“Tyr-176”上的 AKT1 来激活 AKT1，同时磷酸化“Tyr-267”和“Tyr-363”上的 AR，促进其募集至雄激素反应增强子 (ARE)。此外，ACK1 磷酸化“Tyr-287”和 MCF2 上的 WWOX，增强其对 Rho 家族蛋白的鸟嘌呤核苷酸交换因子 (GEF) 活性。此外，ACK1 有助于控制 AXL 受体水平。在癌症中，ACK1 通过负向调节 WWOX 等肿瘤抑制因子和正向调节 AKT1 和 AR 等促生存因子来赋予转移特性并促进肿瘤生长，显示出其在致癌过程中的复杂参与。此外，ACK1 磷酸化 WASP，进一步强调其多样化的信号传导能力。

The specific activity was determined to be ≥ 4 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.

Human

Sf9 insect cells

N-GST

Q07912-1 (G110-W476)

10188  [NCBI]

Approximately 62 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 0.5 mM EDTA, 0.5 mM PMSF, 0.5 mM TCEP.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.